Microbial single-cell growth response at defined carbon limiting conditions.

ABSTRACT: Growth is one of the most fundamental characteristics of life, but detailed knowledge regarding growth at nutrient limiting conditions remains scarce. In recent years progress in microfluidic single-cell analysis and cultivation techniques has given insights into many fundamental growth characteristics such as growth homeostasis, aging and cell division of microbial cells. Using microfluidic single-cell cultivation technologies we examined how single-cell growth at defined carbon conditions, ranging from strongly limiting conditions (0.01 mmol L^-1) to a carbon surplus (100 mmol L^-1), influenced cell-to-cell variability. The experiments showed robust growth of populations at intermediate concentrations and cell-to-cell variability was higher at low and high carbon conc

SUBMITTER: Lindemann D 

PROVIDER: S-EPMC9064036 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature