Single-molecule study on conformational dynamics of M.HhaI.

ABSTRACT: We found that apo DNA methyltransferase M.HhaI under the physiological salt concentration does not possess the structure characterized by X-ray crystallography; instead, it interchanges between prefolded and unfolded states. Only after binding to the substrate, it transforms into a crystal-structure-like state. Flipping rates of its catalytic loop were directly measured.

SUBMITTER: He S 

PROVIDER: S-EPMC9064139 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature