Visualization of the intracellular location and stability of DNA flowers with a label-free fluorescent probe.

ABSTRACT: Rolling circle amplification (RCA) and rolling circle transcription (RCT) can be used to fabricate various structures and organize functional materials for biological applications. The full understanding of the interactions between RCA/RCT-derived structures and live cells is urgently demanded. Here, we present a label-free fluorescent strategy to study the intracellular location and stability of RCA-based DNA flowers in live cells. The DNA flower structures are co-assembled with carbazole-based biscyanine fluorophores, which are DNA detecting molecules and characterized by restriction of intramolecular rotation (RIR) induced strong fluorescent emission. When biscyanine molecules are encapsulated in the DNA flowers via electrostatic attraction, these confined RIR dyes can produce st

SUBMITTER: Wei Y 

PROVIDER: S-EPMC9064242 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature