Comprehensive quali-quantitative profiling of neutral and sialylated O-glycome by mass spectrometry based on oligosaccharide metabolic engineering and isotopic labeling.

ABSTRACT: Mass spectrometry (MS) analysis combined with stable isotopic labeling is of great importance for quantitatively profiling abnormal sialylated O-glycans associated with disease development, but technically hindered by the poor releasing efficiency of O-glycans from glycoprotein or the labile nature of sialic acid residues at glycans. Herein, we developed an isotopic precursor based metabolic amplification and labeling (IPMAL) technique for relative quantitative profiling of the repertoire O-glycans between normal and tumor cells by ESI-MS. Two groups of cells were incubated with peracetylated benzyl-α-N-acetylgalactosamine (Ac₃GalNAc-α-Bn^d0) or a heavy labeled peracetylated benzyl-α-N-acetylgalactosamine (Ac₃GalNAc-α-Bn

SUBMITTER: Nan L 

PROVIDER: S-EPMC9064288 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature