Colorimetric detection of hydrogen peroxide and glucose by exploiting the peroxidase-like activity of papain.

ABSTRACT: Papain, a natural plant protease that exists in the latex of Carica papaya, catalyzes the hydrolysis of peptide, ester and amide bonds. In this work, we found that papain displayed peroxidase-like activity and catalyzed the oxidation of 3,3',5',5'-tetramethylbenzidine (TMB) in the presence of H₂O₂. This results in the formation of a blue colored product with an absorption maximum at 652 nm. The effects of experimental parameters including pH and reaction temperature on catalytic activity of papain were investigated. The increase of absorbance induced by the catalytic effect of papain offers accurate detection of H₂O₂ in the range of 5.00-90.0 μM, along with a detection limit of 2.10 μM. A facile colorimetric method for glucose detection wa

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9064409 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature