Biocementation of soil by calcite/aragonite precipitation using Pseudomonas azotoformans and Citrobacter freundii derived enzymes.

ABSTRACT: Microbial geotechnology is the use of microorganisms and/or their derivatives to alter engineering properties of soil for improving its stability, strength and stiffness. Ureases hydrolyze urea in the soil leading to CaCO₃ precipitation, which binds soil particles together (biocementation). In the present study, nine Egyptian soils were screened for urease-producing bacteria, 15 isolates were obtained, and optimum urease producers were identified. Growth kinetics were measured at different pH values and in the presence of molasses as the sole carbon source. Citrobacter freundii and Pseudomonas azotoformans showed the highest extracellular urease activities of 45.5 ± 3.4 and 54.9 ± 3.5 U ml^-1, respectively. Cell-free supernatants of these isolates mediate

SUBMITTER: Abdel-Aleem H 

PROVIDER: S-EPMC9064560 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature