Bioconjugation of enzyme with silica microparticles: a promising platform for α-amylase partitioning.

ABSTRACT: Here, we report the implementation of α-amylase conjugated silica microparticles for improvement of α-amylase partitioning in a PEG-organic salt-based aqueous two phase system. A direct reduction method was employed for the synthesis of silica microparticles with simultaneous introduction of α-amylase. In this context, we synthesized three different silica α-amylase conjugated microparticles with variation of tetraethyl orthosilicate concentration, and thus the effect of final particle size and enzyme loading on partitioning was also studied. The partition coefficient ratio of α-amylase to Si:α-amylase of 2.186 : 21.701 validated an almost tenfold increase in separation. The microscopic structure of the system was thoroughly investigated in order to understand the extraction mechanism and

SUBMITTER: Karimi M 

PROVIDER: S-EPMC9064637 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature