Biocatalytic transamination in a monolithic flow reactor: improving enzyme grafting for enhanced performance.

ABSTRACT: Transaminases were immobilized onto macrocellular silica monoliths and used for carrying a continuous flow mode transamination reaction. Monoliths were prepared via an emulsion-templated sol-gel method and functionalised by amino-moieties (3-aminopropyl-triethoxysilane, APTES) in order to covalently immobilize the enzymes, using glutaraldehyde as a cross-linking agent. In order to obtain higher performance and improved reproducibility, we investigate the key parameters of APTES functionalisation and of enzyme grafting. Four functionalisation protocols were studied. We show that enhancing the homogeneity of the APTES grafting and controlling the moisture level during functionalisation led to a 3-fold increase in activity as compared to the previously reported data, and greatly improv

SUBMITTER: van den Biggelaar L 

PROVIDER: S-EPMC9064773 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature