MSD-based assays facilitate a rapid and quantitative serostatus profiling for the presence of anti-AAV antibodies.

ABSTRACT: Adeno-associated virus (AAV) vector applications are often limited by capsid-directed humoral immune responses, mainly through neutralizing antibodies (NAbs), which are present throughout the human population due to natural AAV infections. Currently, antibody levels are often quantified via ELISA-based protocols or by cellular NAb assays and less frequently by in vivo NAb assays in mice. These methods need optimization for each serotype and are often not applicable to AAV variants with poor in vitro transduction. To tackle these limitations, we have established Meso Scale Discovery (MSD)-based assays for the quantification of binding antibodies (BAbs) and NAbs against the three most commonly used AAV serotypes, AAV2, AAV8, and AAV9. Both assays detect anti-AAV-IgG_1-3

SUBMITTER: Haar J 

PROVIDER: S-EPMC9065051 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature