Growth factor dependency in mammary organoids regulates ductal morphogenesis during organ regeneration.

ABSTRACT: Signaling pathways play an important role in cell fate determination in stem cells and regulate a plethora of developmental programs, the dysregulation of which can lead to human diseases. Growth factors (GFs) regulating these signaling pathways therefore play a major role in the plasticity of adult stem cells and modulate cellular differentiation and tissue repair outcomes. We consider murine mammary organoid generation from self-organizing adult stem cells as a tool to understand the role of GFs in organ development and tissue regeneration. The astounding capacity of mammary organoids to regenerate a gland in vivo after transplantation makes it a convenient model to study organ regeneration. We show organoids grown in suspension with minimal concentration of Matrigel and in the presence

SUBMITTER: Sahu S 

PROVIDER: S-EPMC9065107 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature