Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons.

ABSTRACT: The Tg(Pax6-cre,GFP)^2Pgr (α-Cre) mouse is a commonly used Cre line thought to be retinal-specific. Using targeted locus amplification (TLA), we mapped the insertion site of the transgene, and defined primers useful to deduce zygosity. Further analyses revealed four tandem copies of the transgene. The insertion site mapped to clusters of vomeronasal and olfactory receptor genes. Using R26R and Ai14 Cre reporter mice, we confirmed retinal Cre activity, but also detected expression in Gα₀⁺ olfactory neurons. Most α-Cre⁺ olfactory neurons do not express Pax6, implicating the influence of neighboring regulatory elements. RT-PCR and buried food pellet test did not detect any effects of the transgene on flanking genes in the nasal mucosa and retina. Tog

SUBMITTER: Fan Y 

PROVIDER: S-EPMC9065156 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature