CRISPR-Cas9 library screening approach for anti-cancer drug discovery: overview and perspectives.

ABSTRACT: CRISPR-Cas9 is a Nobel Prize-winning robust gene-editing tool developed in the last decade. This technique enables a stable genetic engineering method with high precision on the genomes of all organisms. The latest advances in the technology include a genome library screening approach, which can detect survival-essential and drug resistance genes via gain or loss of function. The versatile machinery allows genomic screening for gene activation or inhibition, and targets non-coding sequences, such as promoters, miRNAs, and lncRNAs. In this review, we introduce the emerging high-throughput CRISPR-Cas9 library genome screening technology and its working principles to detect survival and drug resistance genes through positive and negative selection. The technology is compared with other existi

SUBMITTER: Chan YT 

PROVIDER: S-EPMC9065202 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature