A fluorescence sensing platform of theophylline based on the interaction of RNA aptamer with graphene oxide.

ABSTRACT: RNA, with a structure similar to DNA, should exhibit similar behaviors when it interacts with graphene. In this work, we designed a sensing platform of theophylline based on the interaction of an RNA aptamer with graphene oxide (GO) using the fluorescence as a sensing signal. Firstly, quantum dots (QDs) were modified with the selected ssRNA that can be used as an aptamer to recognize the theophylline. The fluorescence of QDs will be quenched in the presence of GO due to the noncovalent assembly between ssRNA aptamer and GO, leading to fluorescence resonance energy transfer (FRET) from QDs to GO, fluorescence "turn-off". Then, in the presence of theophylline, the ssRNA aptamer recognizes theophylline to form a dsRNA-theophylline complex. The weak affinity between the complex and GO makes QD

SUBMITTER: Wu JF 

PROVIDER: S-EPMC9065333 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature