Single-Cell Characterization of Hepatic CD8⁺ T Cells in a Murine Model of Primary Biliary Cholangitis.

ABSTRACT: Primary biliary cholangitis (PBC), an organ-specific autoimmune disease, is characterized by injury to small bile ducts, inflammatory cell infiltrates within the liver, progressive cholestasis, and in some cases, cirrhosis with unclear pathogenesis. We aimed to clarify the importance role of hepatic immunce cells in the pathogenesis of human and experimental PBC.The dominant-negative TGFβ receptor type II transgenic (dnTGFβRII) mice, a well-studied and established murine model of PBC were used to identify changes of immune cells, especially the pathogenic CD8⁺ T cells. The high-throughput single-cell RNA sequencing technology were applied and found functional heterogeneity among the hepatic CD8⁺ T cells subsets in dnTGFβRII mice. CD8⁺ T cells were confirmed

SUBMITTER: Han Y 

PROVIDER: S-EPMC9065443 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature