A facile methodology using quantum dot multiplex labels for tracking co-transfection.

ABSTRACT: Advances in the field of genome engineering demand the development of efficient non-viral transfection agents capable of delivering multiple distinct nucleic acids efficiently to cells (co-transfection). However, current delivery methods result in lower co-transfection efficiency than single plasmid transfections, and the efficiency decreases further with increasing numbers of plasmids. The development of a high-throughput methodology is required for the validation of co-transfection platforms to facilitate independent tracking of not only the multiple DNA plasmids during transfection but also the localisation of transfection agents. This is pivotal to determine the bottlenecks in achieving high transfection efficiencies at various stages of the cell internalisation and plasmid expression

SUBMITTER: Kretzmann JA 

PROVIDER: S-EPMC9065570 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature