Dataset on the content of vitamin D₃ and 25-hydroxyvitamin D₃ in 40 pork (Breitov breed) commodities.

ABSTRACT: This dataset demonstrates the content of vitamin D₃ and 25-hydroxyvitamin D₃ (25OHD₃) in boiled, grilled, and raw pork for both lean and whole cuts including ribs, tenderloin, shoulder, neck, belly, and center chops. Total fat content in raw pork cuts was determined by the gravimetric method. Quantification analysis using reverse phase high performance liquid chromatography with UV-DAD connected. Purification of fatty acids from proteins was performed using a c18 cartridge, resolving vitamin D₃ from its metabolites by subjecting purified samples to polar silica cartridge, purification from sugars by a series of two amino columns, and quantification of vitamin D₃ and 25-hydroxyvitamin D₃ by injecting purified extracts on C18

SUBMITTER: Othman AJ 

PROVIDER: S-EPMC9065703 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature