A strategy for determining the equilibrium constants for heteromeric ion channels in a complex model.

ABSTRACT: Ligand-gated ion channels are oligomers containing several binding sites for the ligands. However, the signal transmission from the ligand binding site to the pore has not yet been fully elucidated for any of these channels. In heteromeric channels, the situation is even more complex than in homomeric channels. Using published data for concatamers of heteromeric cyclic nucleotide-gated channels, we show that, on theoretical grounds, multiple functional parameters of the individual subunits can be determined with high precision. The main components of our strategy are (1) the generation of a defined subunit composition by concatenating multiple subunits, (2) the construction of 16 concatameric channels, which differ in systematically permutated binding sites, (3) the determination of respec

SUBMITTER: Benndorf K 

PROVIDER: S-EPMC9066054 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature