The role of retinoid-related orphan receptor-α in cigarette smoke-induced autophagic response.

ABSTRACT:

Background

Retinoid-related orphan receptor-α (RORα) and autophagy dysregulation are involved in the pathophysiology of chronic obstructive pulmonary disease (COPD), but little is known regarding their association. We investigated the role of RORα in COPD-related autophagy.

Methods

The lung tissues and cells from a mouse model were analyzed for autophagy markers by using western blot analysis and transmission electron microscopy.

Results

Cigarette smoke increased the LC3-II level and decreased the p62 level in whole lung homogenates of a chronic cigarette smoking mouse model. Although cigarette smoke did not affect the levels of p62 in Staggerer mutant mice (RORα^sg/sg), the baseline expression levels of p62 were significantly higher than those in wild type

SUBMITTER: Kim HS 

PROVIDER: S-EPMC9066967 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature