Uncovering the genetic diversity of Giardia intestinalis in isolates from outbreaks in New Zealand.

ABSTRACT:

Background

Giardia intestinalis is one of the most common causes of diarrhoea worldwide. Molecular techniques have greatly improved our understanding of the taxonomy and epidemiology of this parasite. Co-infection with mixed (sub-) assemblages has been reported, however, Sanger sequencing is sometimes unable to identify shared subtypes between samples involved in the same epidemiologically linked event, due to samples showing multiple dominant subtypes within the same outbreak. Here, we aimed to use a metabarcoding approach to uncover the genetic diversity within samples from sporadic and outbreak cases of giardiasis to characterise the subtype diversity, and determine if there are common sequences shared by epidemiologically linked cases that are missed by Sanger sequencing.

Me

SUBMITTER: Ogbuigwe P 

PROVIDER: S-EPMC9066983 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature