Extraction of DNA from complex biological sample matrices using guanidinium ionic liquid modified magnetic nanocomposites.

ABSTRACT: A series of guanidinium ionic liquid modified magnetic chitosan/graphene oxide (GIL-MCGO) nanocomposites have been prepared for DNA extraction via magnetic solid-phase extraction technology. These nanocomposites are of only 20 nanometers in diameter. Single stranded DNA or DNA sodium salts that were absorbed by GIL-MCGO could be quickly collected by an external magnet and extracted. The DNA extraction efficiency of 11 GIL-MCGO nanocomposites was evaluated using NanoDrop. Factors that could impact the DNA extraction process, such as pH, temperature, extraction time, and ionic strength were systematically investigated via single-factor experimental analysis. Under the optimum extraction conditions, a maximum DNA extraction capacity of 233.0 ± 0.4 mg g^-1 of GIL-MCGO n

SUBMITTER: Liu M 

PROVIDER: S-EPMC9067247 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature