Rapid electrochemical quantification of trace Hg²⁺ using a hairpin DNA probe and quantum dot modified screen-printed gold electrodes.

ABSTRACT: Rapid, simple, sensitive and specific approaches for mercury(ii) (Hg²⁺) detection are essential for toxicology assessment, environmental protection, food analysis and human health. In this study, a ratiometric hairpin DNA probe based electrochemical biosensor, which relies on hairpin DNA probes conjugated with water-soluble and carboxyl functionalized quaternary Zn-Ag-In-S quantum dot (QD) on screen-printed gold electrodes (SPGE), referred to as the HP-QDs-SPGE electrochemical biosensor in this study, was developed for Hg²⁺ detection. Based on the "turn-off" reaction of a hairpin DNA probe binding with a mismatched target and Hg²⁺ through the formation of T-Hg²⁺-T coordination, the HP-QDs-SPGE electrochemical biosensor can rapidly quantify trace

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9067431 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature