Synthetic biology and bioelectrochemical tools for electrogenetic system engineering.

ABSTRACT: Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, the use of electrogenetic systems is limited by poor activity, tunability, and standardization. In this work, we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Last, we demonstrated electrochemical activ

SUBMITTER: Lawrence JM 

PROVIDER: S-EPMC9067924 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature