MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna.

ABSTRACT: Lipids play various essential roles in the physiology of animals. They are also highly dependent on cellular metabolism or status. It is therefore crucial to understand to which extent animals can stabilize their lipid composition in the presence of external stressors, such as chemicals that are released into the environment. We developed a MALDI MS imaging workflow for two important aquatic model organisms, the zebrafish (Danio rerio) and water flea (Daphnia magna). Owing to the heterogeneous structure of these organisms, developing a suitable sample preparation workflow is a highly non-trivial but crucial part of this work and needs to be established first. Relevant parameters and practical considerations in order to preserve tissue structure and composition in tissue sections are discus

SUBMITTER: Schirmer E 

PROVIDER: S-EPMC9068711 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature