Nanopore Sequencing for Detection and Characterization of Phosphorothioate Modifications in Native DNA Sequences.

ABSTRACT: Bacterial DNA is subject to various modifications involved in gene regulation and defense against bacteriophage attacks. Phosphorothioate (PT) modifications are protective modifications in which the non-bridging oxygen in the DNA phosphate backbone is replaced with a sulfur atom. Here, we expand third-generation sequencing techniques to allow for the sequence-specific mapping of DNA modifications by demonstrating the application of Oxford Nanopore Technologies (ONT) and the ELIGOS software package for site-specific detection and characterization of PT modifications. The ONT/ELIGOS platform accurately detected PT modifications in a plasmid carrying synthetic PT modifications. Subsequently, studies were extended to the genome-wide mapping of PT modifications in the Salmonella enterica

SUBMITTER: Wadley T 

PROVIDER: S-EPMC9069010 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature