Single-cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy.

ABSTRACT:

Background

Hymenoptera stings can induce dysregulated inflammation and immediate hypersensitivity reactions including anaphylaxis. However, the molecular mechanisms underlying peripheral immune responses during Hymenoptera venom allergy (HVA) remain elusive.

Methods

Here we determined the single-cell transcriptomic profiling from highly heterogeneous peripheral blood cells in patients with HVA through unbiased single-cell RNA sequencing and multiple models of computational analyses.

Results

Through clustering analysis by uniform manifold approximation and projection, we revealed an increased number of monocytes in the acute phase and identified innate immune responses, leukocyte activation, and cellular detoxification as the main involved biological processes. We used

SUBMITTER: Chao WC 

PROVIDER: S-EPMC9069361 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature