A spherical poly(acrylic acid) brush-enzyme block with high catalytic capacity for signal amplification in digital biological assays.

ABSTRACT: Ultrasensitive determination of some ultra-low abundance biological molecules closely related to diseases is currently a wide concern and urgent issue to be addressed. Here, a spherical poly(acrylic acid)-alkaline phosphatase (SP-AKP) signal amplification block using spherical poly(acrylic acid) brush nanoparticles (SP) as the immobilized carriers was designed and synthesized optimally first. The results show that a single SP-AKP with high enzyme binding capacity and high catalytic ability (up to about 4800 effective free AKP per SP-AKP) has much greater fluorescence signal amplification ability than a single free AKP or SiO₂-COOH-AKP. Then, a droplet generation microfluidic chip was prepared successfully, and the SP-AKP was loaded and confined in a 14 pL droplet by adjusting it

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9069456 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature