Raman cell imaging with boron cluster molecules conjugated with biomolecules.

ABSTRACT: Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B-H stretching vibrations of two types of simple icosahedral boron clusters, ortho-carborane 3 and closo-dodecaborate 4 appeared at approximately 2450-2700 cm^-1, and did not overlap with those of cellular components. Although ortho-carborane 3 possesses a possible property as a Raman probe, it was difficult to measure Raman imaging in the cell due to its poor water solubility. In fact, ortho-carborane derivative 6, which internally has an alkyne moiety, exhibited very weak Raman signals of the C[triple bond, length as m-dash]C stretching and the B-H stretching vibrations were barely detected at a 400 ppm boron concentration in HeLa cells. In

SUBMITTER: Mochizuki M 

PROVIDER: S-EPMC9069464 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature