Single-Neuron Labeling in Fixed Tissue and Targeted Volume Electron Microscopy.

ABSTRACT: The structural complexity of nervous tissue makes it very difficult to unravel the connectivity between neural elements at different scales. Numerous methods are available to trace long-range projections at the light microscopic level, and to identify the actual synaptic connections at the electron microscopic level. However, correlating mesoscopic and nanoscopic scales in the same cell, cell population or brain region is a problematic, laborious and technically demanding task. Here we present an effective method for the 3D reconstruction of labeled subcellular structures at the ultrastructural level, after single-neuron labeling in fixed tissue. The brain is fixed by intracardial perfusion of aldehydes and thick vibratome sections (250 μm) are obtained. Single cells in these vibratome sec

SUBMITTER: Turegano-Lopez M 

PROVIDER: S-EPMC9070053 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature