Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cells.

ABSTRACT: Inositol plays a significant role in cellular function and signaling. Studies in yeast have demonstrated an "inositol-less death" phenotype, suggesting that inositol is an essential metabolite. In yeast, inositol synthesis is highly regulated, and inositol levels have been shown to be a major metabolic regulator, with its abundance affecting the expression of hundreds of genes. Abnormalities in inositol metabolism have been associated with several human disorders. Despite its importance, very little is known about the regulation of inositol synthesis and the pathways regulated by inositol in human cells. The current study aimed to address this knowledge gap. Knockout of ISYNA1 (encoding myo-inositol-3-P synthase 1) in HEK293T cells generated a human cell line that is deficient in de novo i

SUBMITTER: Suliman M 

PROVIDER: S-EPMC9070113 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature