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Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte.

ABSTRACT: With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO^- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO^- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.

SUBMITTER: Wu L

PROVIDER: S-EPMC9070123 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte.

Wu Luling L Gardiner Jordan E JE Kumawat Lokesh K LK Han Hai-Hao HH Guo Ruiying R Li Xin X He Xiao-Peng XP Elmes Robert B P RBP Sedgwick Adam C AC Bull Steven D SD James Tony D TD

RSC advances 20190827 45

With this research we set out to develop a number of coumarin-based 'AND' logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a s ...[more]

PMID: 35530981

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