Isolation and identification of an antioxidant collagen peptide from skipjack tuna (Katsuwonus pelamis) bone.

ABSTRACT: To date, many researchers have developed active components that are derived from seafood processing for the purposes of healthcare. Here, an antioxidant collagen peptide was obtained from skipjack tuna (Katsuwonus pelamis) bone by using a combination of trypsin and chymotrypsin as the catalyst. The amino acid sequence of the peptide was identified as Ser-Ser-Gly-Pro-Pro-Val-Pro-Gly-Pro-Met-Gly-Pro-Met-Gly-Pro-Arg (SSGPPVPGPMGPMGPR) by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) analysis. We found that the as-prepared collagen peptide can efficiently scavenge DPPH radical (IC₅₀ 3.149 mM), superoxide anion radical (IC₅₀ 3.803 mM) and ABTS radical (IC₅₀ 9.489 mM). In addition, it has been fo

SUBMITTER: Ding D 

PROVIDER: S-EPMC9070664 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature