A method for the selective depletion of microglia in the dorsal hippocampus in the juvenile rat brain.

ABSTRACT:

Background

To understand the role of microglia in brain function and development, methods have emerged to deplete microglia throughout the brain. Liposome-encapsulated clodronate (LEC) can be infused into the brain to deplete microglia in a brain-region and time-specific manner.

New method

This study validates methodology to deplete microglia in the rat dorsal hippocampus (dHP) during a specific period of juvenile development. Stereotaxic surgery was performed to infuse LEC at postnatal day (P) 16 or 19 into dHP. Rat brains were harvested at various ages to determine specificity of infusion and duration of depletion.

Results

P19 infusion of LEC into dHP with a 27G syringe depleted microglia in dHP subregions CA1, dentate gyrus (DG), and CA3 from P24-P30. There was als

SUBMITTER: Hall MB 

PROVIDER: S-EPMC9070732 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature