A restricted access molecularly imprinted polymer coating on metal-organic frameworks for solid-phase extraction of ofloxacin and enrofloxacin from bovine serum.

ABSTRACT: A restricted access molecularly imprinted polymer (RAMIP) crosslinked with bovine serum albumin (BSA) was prepared on the surface of the mesoporous UiO-66-NH₂ metal-organic framework (MOF). The surface morphology, imprinting behavior, and protein exclusion properties of UiO-66-NH₂@RAMIP@BSA were investigated. The maximum adsorption capacity was 50.55 mg g^-1 for ofloxacin, with a 99.4% protein exclusion rate. Adsorption equilibrium was reached in 9 min. Combined with RP-HPLC, a solid-phase extraction column filled with UiO-66-NH₂@RAMIP@BSA was used to selectively enrich and analyze ofloxacin and enrofloxacin antibiotics from bovine serum with recoveries of 93.7-104.2% with relative standard deviations of 2.0-4.5% (n = 3). The linear range a

SUBMITTER: Sun Z 

PROVIDER: S-EPMC9070776 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature