Zonal rotor centrifugation revisited: new horizons in sorting nanoparticles.

ABSTRACT: Density gradient centrifugation is an effective method for the isolation and purification of small particles. Hollow rotors capable of hosting density gradients replace the need for centrifuge tubes and therefore allow separations at large scales. So far, zonal rotors have been used for biological separations ranging from the purification of whole cells down to serum proteins. We demonstrate that the high-resolution separation method opens up exciting perspectives apart from biology, namely in sorting mixtures of synthetic nanoparticles. Loading and unloading, while the rotor is spinning, avoids perturbations during acceleration and deceleration periods, and thus makes a vital contribution to sorting accuracy. Nowadays one can synthesize nanoscale particles in a wide variety of composition

SUBMITTER: Pluisch CS 

PROVIDER: S-EPMC9070787 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature