Rapid, label-free pathogen identification system for multidrug-resistant bacterial wound infection detection on military members in the battlefield.

ABSTRACT: US military service members experiencing combat-related wounds have higher risk of infection by multidrug-resistant bacteria. The gold standard culture-based antimicrobial susceptibility testing (AST) is not feasible in the battlefield environment. Thus, a rapid deployable system for bacteria identification and AST directly from wound sample is urgently needed. We report the potential of a Rapid, Label-free Pathogen Identification (RAPID) diagnostic system based on ATR-FTIR method to detect and distinguish multi-drug resistant strains for six different species in the ESKAPEE group. Our RAPID system combines sample processing on-broad to isolate and enrich bacteria cells from wound sample, ATR-FTIR measurement to detect antimicrobial-induced bacterial cell spectral changes, and machine lear

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9070933 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature