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Designing dendronic-Raman markers for sensitive detection using surface-enhanced Raman spectroscopy.

ABSTRACT: Surface-Enhanced Raman Spectroscopy (SERS) is well-established as a tool for bio-diagnostics but is often limited by analyte sensitivity and the need for specialized substrates. Signal enhancement can be achieved by attaching multiple Raman markers to a single analyte. Dendronic frameworks with multiple Raman markers attached to the periphery offer an opportunity to examine this idea. In this article, dendrons with thiophenol groups on their periphery were synthesized and tested as a SERS analyte. For this study, simple gold nanoparticles (∼60 nm) were used as a substrate. A 10² fold enhancement in detection was observed upon going from a mono-thiophenol (MT) to a tetra-thiophenol (TT). Dendronic Raman markers increased the probability of SERS occurrence at lower concentrations when compared to a single Raman active molecule. This strategy extends the applicability of SERS, as these analyte molecules can be just mixed or drop-casted on any kind of SERS substrate.

SUBMITTER: Jain P

PROVIDER: S-EPMC9071040 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Designing dendronic-Raman markers for sensitive detection using surface-enhanced Raman spectroscopy.

Jain Priyanka P Patra Robi Sankar RS Rajaram Sridhar S Narayana Chandrabhas C

RSC advances 20190909 48

Surface-Enhanced Raman Spectroscopy (SERS) is well-established as a tool for bio-diagnostics but is often limited by analyte sensitivity and the need for specialized substrates. Signal enhancement can be achieved by attaching multiple Raman markers to a single analyte. Dendronic frameworks with multiple Raman markers attached to the periphery offer an opportunity to examine this idea. In this article, dendrons with thiophenol groups on their periphery were synthesized and tested as a SERS analyt ...[more]

PMID: 35530472

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Project description:We report the demonstration of an optofluidic surface enhanced Raman spectroscopy (SERS) device that leverages a nanoporous microfluidic matrix to improve the SERS detection performance by more than two orders of magnitude as compared to a typical open microfluidic channel. Although it is a growing trend to integrate optical biosensors into microfluidic channels, this basic combination has been detrimental to the sensing performance when applied to SERS. Recently, however, synergistic combinations between microfluidic functions and photonics (i.e., optofluidics) have been implemented that improve the detection performance of SERS. Conceptually, the simplest optofluidic SERS techniques reported to date utilize a single nanofluidic channel to trap nanoparticle-analyte conjugates as a method of preconcentration before detection. In this work, we leverage this paradigm while improving upon the simplicity by forming a 3D nanofluidic network with packed nanoporous silica microspheres in a microfluidic channel; this creates a concentration matrix that traps silver nanoclusters and adsorbed analytes into the SERS detection volume. With this approach, we are able to achieve a detection limit of 400 attomoles of Rhodamine 6G after only 2 min of sample loading with high chip-to-chip repeatability. Due to the high number of fluidic paths in the nanoporous channel, this approach is less prone to clogging than single nanofluidic inlets, and the loading time is decreased compared to previous reports. In addition, fabrication of this microsystem is quite simple, as nanoscale fabrication is not necessary. Finally, integrated multimode fiber optic cables eliminate the need for optical alignment, and thus the device is relevant for portable and automated applications in the field, including point-of-sample and point-of-care detection. To illustrate a relevant field-based application, we demonstrate the detection of 12 ppb of the organophosphate malathion in water using the nanofluidic SERS microsystem.

Dataset Information

Designing dendronic-Raman markers for sensitive detection using surface-enhanced Raman spectroscopy.

Publications

Designing dendronic-Raman markers for sensitive detection using surface-enhanced Raman spectroscopy.

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