Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme.

ABSTRACT: We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N-isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction parameters such as monomer concentration and reaction temperature on the microgel size and size distribution is performed and immobilization yields are determined. Microgels exhibiting hydrodynamic diameters between 100 nm and 1 μm and narrow size distribution could be synthesized while about 31-44% of the enzyme present in the initial reaction mixture can be immobilized. Successful immobilization including a verification of enzymatic activity of the microgels is achieved for glucose oxidase (

SUBMITTER: Reinicke S 

PROVIDER: S-EPMC9071056 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature