Identification of a WRKY transcriptional activator from Camellia sinensis that regulates methylated EGCG biosynthesis.

ABSTRACT: Naturally occurring methylated catechins, especially methylated EGCG in tea leaves are known to have many health benefits. Although the genes involved in methylated EGCG biosynthesis have been studied extensively, the transcriptional factors controlling methylated EGCG biosynthesis are still poorly understood. In the present study, a WRKY domain-containing protein termed CsWRKY57like was identified, which belongs to group IIc of the WRKY family, and contains one conserved WRKY motif. CsWRKY57like was found to localize in the nucleus, function as a transcriptional activator, and its expression positively correlated with methylated EGCG level. In addition, CsWRKY57like activated the transcription of three genes related to methylated EGCG biosynthesis, including CCoAOMT, CsLAR, and CsDFR by s

SUBMITTER: Luo Y 

PROVIDER: S-EPMC9071374 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature