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Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels.

ABSTRACT: New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Furthermore, hydrogels with the introduction of disulfide bonds viaDDA-4 could be chemically degraded by dithiothreitol. Our results provided a facile approach for the direct construction of virus-based hydrogels.

SUBMITTER: Ma D

PROVIDER: S-EPMC9071817 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels.

Ma Dejun D Chen Zhuoyue Z Yi Long L Xi Zhen Z

RSC advances 20190917 50

New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Furthermore, hydrogels with the introduction of disulfide bonds <i>via</i>DDA-4 could be chemically degraded by dithiothreitol. Our results provided a facile approach for the direct construction of virus ...[more]

PMID: 35528434

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