Efficient release of immunocaptured cells using coiled-coils in a microfluidic device.

ABSTRACT: Label-free and affinity-based cell separation allows highly specific cell capture through simple procedures, but it remains a major challenge to efficiently release the captured cells without changing their structure, phenotype, and function. We report a microfluidic platform for label-free immunocapture of target cells and efficient release of the cells with minimal biochemical and biophysical perturbations. The method capitalizes on self-assembly of a pair of heterodimerizing coiled-coils, A and B. Target cells are captured in microchannels functionalized with an antibody and A and efficiently released by a liquid flow containing B-PEG (a conjugate of B and polyethylene glycol) at a controlled, low shear stress. The released cells have no antibodies attached or endogenous surface molecul

SUBMITTER: Zhang M 

PROVIDER: S-EPMC9071837 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature