Molecular Hydrogen Inhibits Colorectal Cancer Growth via the AKT/SCD1 Signaling Pathway.

ABSTRACT:

Objective

Molecular hydrogen (H₂) has been considered a potential therapeutic target in many cancers. Therefore, we sought to assess the potential effect of H₂ on colorectal cancer (CRC) in this study.

Methods

The effect of H₂ on the proliferation and apoptosis of RKO, SW480, and HCT116 CRC cell lines was assayed by CCK-8, colony formation, and flow cytometry assays. The effect of H₂ on tumor growth was observed in xenograft implantation models (inhalation of 67% hydrogen two hours per day). Western blot and immunohistochemistry analyses were performed to examine the expression of p-PI3K, PI3K, AKT, pAKT, and SCD1 in CRC cell lines and xenograft mouse models. The expression of SCD1 in 491 formalin-fixed, paraffin-embedded CRC speci

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9071919 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature