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Nanopipette-assisted single cell metabolic glycan labeling.

ABSTRACT: Here, we report a single cell glycan labeling strategy by combining nanoscale intracellular glass electrodes with bioorthogonal reaction. With the tip diameter less than 100 nm, the nanopipette electrode can be spatially controlled to inject artificial monosaccharides into single living cells with minimal invasion. The injection process can be precisely regulated by electroosmotic flow inside the nanopipette, and fluorescence labeling of sialic acid at single cell level is achieved.

SUBMITTER: Zhou ZR 

PROVIDER: S-EPMC9072180 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Nanopipette-assisted single cell metabolic glycan labeling.

Zhou Ze-Rui ZR   Wang Xiao-Yuan XY   Lv Jian J   Chen Bin-Bin BB   Tang Yi-Bin YB   Qian Ruo-Can RC  

RSC advances 20190927 53

Here, we report a single cell glycan labeling strategy by combining nanoscale intracellular glass electrodes with bioorthogonal reaction. With the tip diameter less than 100 nm, the nanopipette electrode can be spatially controlled to inject artificial monosaccharides into single living cells with minimal invasion. The injection process can be precisely regulated by electroosmotic flow inside the nanopipette, and fluorescence labeling of sialic acid at single cell level is achieved. ...[more]

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