Visualizing the lipid dynamics role in infrared neural stimulation using stimulated Raman scattering.

ABSTRACT: Infrared neural stimulation (INS) uses pulsed infrared light to yield label-free neural stimulation with broad experimental and translational utility. Despite its robust demonstration, INS's mechanistic and biophysical underpinnings have been the subject of debate for more than a decade. The role of lipid membrane thermodynamics appears to play an important role in how fast IR-mediated heating nonspecifically drives action potential generation. Direct observation of lipid membrane dynamics during INS remains to be shown in a live neural model system. We used hyperspectral stimulated Raman scattering microscopy to study biochemical signatures of high-speed vibrational dynamics underlying INS in a live neural cell culture model. The findings suggest that lipid bilayer structural changes occu

SUBMITTER: Adams WR 

PROVIDER: S-EPMC9072573 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature