Single-molecule phospholipase A₂ becomes processive on melittin-induced membrane deformations.

ABSTRACT: While it is established that the topology of lipid membranes plays an important role in biochemical processes, few direct observations exist regarding how the membranes are actively restructured and its consequences on subsequent reactions. In this work, we investigated how the two major components of bee venom, melittin and phospholipase A₂ (PLA₂), achieve activation by such membrane remodeling. Their membrane-disrupting functions have been reported to increase when both are present, but the mechanism of this synergism had not been established. Using membrane reconstitution, we found that melittin can form large-scale membrane deformities upon which PLA₂ activity is 25-fold higher. Tracking of single-molecule PLA₂ revealed that its processive be

SUBMITTER: Jepson TA 

PROVIDER: S-EPMC9072580 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature