Transcriptome-Wide Dynamics of m⁶A Methylation in Tumor Livers Induced by ALV-J Infection in Chickens.

ABSTRACT: Avian Leukosis Virus Subgroup J (ALV-J) is a tumorigenic virus with high morbidity and rapid transmission. N6-methyladenosine (m⁶A) is a common epigenetic modification that may be closely related to the pathogenicity of ALV-J. Currently, there are no reports on whether m⁶A modification is related to ALV-J induced tumor formation. In this study, we used methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to examine the differences in m⁶A methylation and gene expression in normal livers and ALV-J-induced tumor livers systematically, with functional enrichment and co-expression analysis. The results identified 6,541 m⁶A methylated peaks, mainly enriched in CDS, and more than 83% of the transcripts contained 1-2 m

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC9072629 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature