F₁-ATPase Rotary Mechanism: Interpreting Results of Diverse Experimental Modes With an Elastic Coupling Theory.

ABSTRACT: In this chapter, we review single-molecule observations of rotary motors, focusing on the general theme that their mechanical motion proceeds in substeps with each substep described by an angle-dependent rate constant. In the molecular machine F1-ATPase, the stepping rotation is described for individual steps by forward and back reaction rate constants, some of which depend strongly on the rotation angle. The rotation of a central shaft is typically monitored by an optical probe. We review our recent work on the theory for the angle-dependent rate constants built to treat a variety of single-molecule and ensemble experiments on the F₁-ATPase, and relating the free energy of activation of a step to the standard free energy of reaction for that step. This theory, an elastic molecu

SUBMITTER: Volkan-Kacso S 

PROVIDER: S-EPMC9072658 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature