Non-detergent isolation of a cyanobacterial photosystem I using styrene maleic acid alternating copolymers.

ABSTRACT: Photosystem I (PSI) from the thermophilic cyanobacterium Thermosynechococcus elongatus (Te) is the largest membrane protein complex to have had its structure solved by X-ray diffraction. This trimeric complex has 36 protein subunits, over 380 non-covalently bound cofactors and a molecular weight of ∼1.2 MDa. Previously, it has been isolated and characterized in a detergent micelle using the non-ionic detergent n-dodecyl-β-d-maltoside (DDM). We have now succeeded in isolating this complex without the use of detergents, using styrene-maleic acid (SMA) alternating copolymer. Intriguingly, a partially esterified copolymer formulation (SMA 1440, Cray Valley) was found to be most efficient in cyanobacterial thylakoid membranes. A host of biochemical, biophysical and functional assa

SUBMITTER: Brady NG 

PROVIDER: S-EPMC9072662 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature