The Role of Autophagy in the Mother-to-Child Transmission of Pregnant Women With a High Level of HBV DNA.

ABSTRACT:

Background

Mother-to-child transmission (MTCT) is the most common propagation mode of hepatitis B virus (HBV) transmission. Exploring the mechanisms of HBV MTCT is the key to protect infant from infection. In this study, we aim to clarify the important role of autophagy complicated in HBV MTCT.

Methods

A total of 169 placental samples were collected in this study, includes 144 HBV positive pregnant women and 25 normal pregnant women. In vitro, JEG-3 cells were treated with serum contained different HBV viral loads. Electron microscope was used to observed the number of autophagosome. RT-qPCR and western blotting were used to measure the expression level of autophagy relative genes and proteins respectively. Immunofluorescence was used to analyzed the expression of LC-

SUBMITTER: Gao H 

PROVIDER: S-EPMC9072787 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature