CRISPR/Cas9 mediated sex-ratio distortion by sex specific gene editing in Aedes aegypti.

ABSTRACT: Aedes aegypti is a principal vector for several viruses including dengue virus, chikungunya virus and zika virus. Economic burden of mosquito-borne diseases, relative failure of traditional control strategies and the resistance development against insecticides enforces towards genetic manipulation of Ae. aegypti. Hence, a key gene doublesex (Aedsx) which regulate sex differentiation and alternatively splices to form male and female specific transcripts (Aedsx^M and Aedsx^F ). CRISPR/Cas9 technique was employed to sex specifically disrupt the female-specific isoforms, Aedsx^F1 and Aedsx^F2 , both of which were shown to be expressed only in female mosquitoes. Targeting of dsx^F

SUBMITTER: Ranian K 

PROVIDER: S-EPMC9073027 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature