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Unveiling the effect of 11-MUA coating on biocompatibility and catalytic activity of a gold-core cerium oxide-shell-based nanozyme.

ABSTRACT: The biocompatibility and catalytic activity of nanomaterials exhibiting biological enzyme-like functions (nanozymes) are controlled by shape, size, composition, and surface capping molecules. Although synthesis of multifunctional nanozymes for multiple applications has shown tremendous attraction among researchers worldwide, often their biocompatibility is compromised. In this work, we report the replacement of CTAB by 11-MUA from the surface of a Au-core CeO₂-shell NP-based nanozyme studied for exhibiting multiple enzyme-like activities such as peroxidase, catalase, and superoxide dismutase. We compared the biocompatibility and enzyme-like activities of CTAB coated Au-core CeO₂-shell NPs (CSNPs) before and after 11-MUA coating. The catalytic reaction mechanism of peroxidase-like activity of CTAB coated CSNPs was found to be the "Random Bi-Bi", which also remained unaltered after removal of surface CTAB with 11-MUA. The other kinetic parameters, K _m and V _max values, of 11-MUA coated CSNPs were found to be comparable to the CTAB coated NPs.

SUBMITTER: Jain V

PROVIDER: S-EPMC9073341 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Unveiling the effect of 11-MUA coating on biocompatibility and catalytic activity of a gold-core cerium oxide-shell-based nanozyme.

Jain Vidhi V Bhagat Stuti S Singh Mandeep M Bansal Vipul V Singh Sanjay S

RSC advances 20191016 57

The biocompatibility and catalytic activity of nanomaterials exhibiting biological enzyme-like functions (nanozymes) are controlled by shape, size, composition, and surface capping molecules. Although synthesis of multifunctional nanozymes for multiple applications has shown tremendous attraction among researchers worldwide, often their biocompatibility is compromised. In this work, we report the replacement of CTAB by 11-MUA from the surface of a Au-core CeO<sub>2</sub>-shell NP-based nanozyme ...[more]

PMID: 35529115

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Project description:Cerium dioxide (CeO2) and silicon dioxide (SiO2) nanoparticles are of widespread use in modern life. This means that human beings are markedly exposed to them in their everyday life. Once passing biological barriers, these nanoparticles are expected to interact with endothelial cells, leading to systemic alterations with distinct influences on human health. In the present study we observed the metabolic impact of differently sized CeO2 (8 nm; 35 nm) and SiO2 nanoparticles (117 nm; 315 nm) on immortalized human microvascular (HMEC-1) and primary macrovascular endothelial cells (HUVEC), with particular focus on the CeO2 nanoparticles. The characterization of the CeO2 nanoparticles in cell culture media with varying serum content indicated a steric stabilization of nanoparticles due to interaction with proteins. After cellular uptake, the CeO2 nanoparticles were localized around the nucleus in a ring-shaped manner. The nanoparticles revealed concentration and time, but no size-dependent effects on the cellular adenosine triphosphate levels. HUVEC reacted more sensitively to CeO2 nanoparticle exposure than HMEC-1. This effect was also observed in relation to cytokine release after nanoparticle treatment. The CeO2 nanoparticles exhibited a specific impact on the release of diverse proteins. Namely, a slight trend towards pro-inflammatory effects, a slight pro-thrombotic impact, and an increase of reactive oxygen species after nanoparticle exposure were observed with increasing incubation time. For SiO2 nanoparticles, concentration- and time-dependent effects on the metabolic activity as well as pro-inflammatory reactions were detectable. In general, the effects of the investigated nanoparticles on endothelial cells were rather insignificant, since the alterations on the metabolic cell activity became visible at a nanoparticle concentration that is by far higher than those expected to occur in the in vivo situation (CeO2 nanoparticles: 100 µg/mL; SiO2 nanoparticles: 10 µg/mL).

Dataset Information

Unveiling the effect of 11-MUA coating on biocompatibility and catalytic activity of a gold-core cerium oxide-shell-based nanozyme.

Publications

Unveiling the effect of 11-MUA coating on biocompatibility and catalytic activity of a gold-core cerium oxide-shell-based nanozyme.

Similar Datasets

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